PAM-2100——野外光合作用研究的首选仪器
Schreiber教授因发明PAM系列调制叶绿素荧光仪而获得首届国际光合作用协会（ISPR）创新奖

1983年，WALZ公司首席科学家、德国乌兹堡大学的Ulrich Schreiber教授设计制造了全世界第一台调制荧光仪——PAM-101/102/103，使在自然光下测量叶绿素荧光成为现实，解决了科学界近50年的技术瓶颈。PAM-101/102/103迅速在植物生理、生态、农学、林学、水生生物学等领域得到广泛应用，出版了大量高水平研究文献。但该仪器比较笨重，不易带到野外。

1992年，WALZ公司首席科学家、调制荧光仪发明人、德国乌兹堡大学的Ulrich Schreiber教授设计制造了全世界第一台便携式调制荧光仪——PAM-2000，并且在植物生理生态学等科研领域得到广泛应用，此后十几年中成为全球最畅销的调制荧光仪。

2003年，WALZ公司在保留PAM-2000所有功能和优点的基础上，结合最新技术，将PAM-2000升级到了PAM-2100。

调制叶绿素荧光仪系统描述
PAM-2100采用了独特的调制技术和饱和脉冲技术，从而可以通过选择性的原位测量叶绿素荧光来检测植物光合作用的变化。PAM-2100的调制测量光足够低，可以只激发色素的本底荧光而不引起任何的光合作用，从而可以真实的记录基础荧光Fo。PAM-2100具有很强的灵敏度和选择性，使其即使在很强的、未经滤光片处理的环境下（如全日照甚至是10000 μmol m^-2 s^-1的饱和光强下）也可测定荧光产量而不受到干扰。因此，PAM-2100不但适合在实验室人工控制的环境下测量，还可以在自然环境中甚至是强烈的全光照条件下开展野外科学研究。

PAM-2100是非常便携、强大的测量系统，它将各种光学和电子元件组装在一个24 cm×10.5 cm×11 cm的外壳中。测量光由655 nm的发光二极管（LED）发出，可在低频（600 Hz）和高频（20 kHz）间自动切换。光化光（光合生物实际可吸收利用进行光合作用的可见光）由卤素灯（白光）或红光LED（655 nm）提供。远红光（735 nm，促进光系统I迅速消耗掉在PQ处累积的电子）由LED发出。

PAM-2100的按键操作非常简单。基础测量只需单健操作。数据在内置电脑中自动分析、存储并且在显示屏上显示。除了“参数窗”外，在“动力学窗”还可显示曲线的实时变化。

PAM-2100利用光纤进行信号传输。光适应叶夹2030-B（专利产品）上配备微型光量子/温度传感器，可在记录荧光信号的同时，同步记录光合有效辐射（PAR）和温度变化。

PAM-2100内设10个标准Run（预先编好的间隔一定时间并按一定顺序执行特定命令的程序），用户只需一次按键就可进行复杂的实验。用户还可对这些标准Run进行编辑得到自己的User-Run（数量不限），来满足特殊的实验需要。
PAM-2100主机可以直接连接电脑（圆口）键盘，在野外现场，可以根据实验需要，不需电脑就可以进行特殊程序的编辑。

PAM-2100还可以设定单机操作软件DA-2100自动间隔一定时间执行某个Run或User-Run，而Run是可以无限扩展的，因此，可以说PAM-2100的功能几乎可以无限扩展。只要将主机和叶夹（均可固定在三角架上）固定好，按一次按键，（人不在现场看守）仪器可以自动进行非常复杂的测量过程。

此外，PAM-2100主机还可以连接电脑显示器或投影仪放大显示，非常适合进行教学使用。

调制叶绿素荧光仪特点
1) 声誉卓著的PAM-2000的升级版
2) 精巧、准确、迅速、操作简便的高级光合作用检测设备
3) 可单机操作（采用内置电脑，DA-2100软件记录），可连接外置电脑操作（Windows操作软件PamWin）
4) 便携式设计，带大屏幕液晶显示屏（可显示曲线变化）和20个按键
5) 强大的数据收集、分析和存贮功能
6) 可以预先编写和设定程序，进行特殊研究目的测量
7) 内置锂电池可满足长时间野外工作需要，并可连接外置12 V电池
8) 多种叶夹可供选择，专利设计的光适应叶夹2030-B可同时记录PAR和温度变化
9) 光源选择：自然光，内置光源（提供测量光、光化光、饱和脉冲和远红光），可选外置卤素灯光源（特别适合野外研究）

调制叶绿素荧光仪功能
1) 可测荧光诱导曲线的快速上升动力学O-I-D-P相和O-J-I-P相
2) 可测荧光诱导曲线的慢速下降动力学并进行淬灭分析（Fo, Fm, Fv/Fm, F, Fm, Fo’, dF/Fm’, qP, qN, NPQ, rETR等）
3) 可测光响应曲线和快速光曲线（RLC）
4) 仪器内置一系列标准实验（Run1～Run10），用户可对其进行编辑建立自己的User-Run
5) 可在线检测植物、微藻、地衣、苔藓等的光合作用变化
6) 单机操作功能强大，特别适合野外操作，实验室内单机操作时可连接电脑显示器或投影仪放大显示

调制叶绿素荧光仪应用领域
仪器设计特别适合野外使用，可用于研究光合作用机理、各种环境因子（光、温、营养等）对植物生理状态的影响、植物抗逆性（干旱、冷、热、涝、UV、病毒、污染、重金属等）、植物的长期生态学变化等。在植物生理学、植物生态学、植物病理学、农学、林学、园艺学、水生生物学、环境科学、毒理学、微藻生物技术、极地植物光合作用研究等领域有着广泛应用。

调制叶绿素荧光仪10个标准Run
Run 1：测量实际量子产量Yield（ΔF/Fm’）
Run 2：测量最大量子产量Fv/Fm
Run 3：记录诱导曲线并进行淬灭分析（采点率10 ms/点）
Run 4：记录诱导曲线并进行淬灭分析（采点率30 ms/点）
Run 5：qN 的驰豫动力学
Run 6：快速诱导动力学O-I-D-P相（线性时间）
Run 7：快速诱导动力学O-J-I-P相（对数时间）
Run 8：光响应曲线（需76 min）（稍加编辑即可测量快速光曲线）
Run 9：光响应曲线（需33 min）（稍加编辑即可测量快速光曲线）
Run 10：仪器自检

用户可根据实验需要，自行修改或编制程序；如需帮助，请来电咨询！

调制叶绿素荧光仪技术参数
测量光：红色发光二极管（LED），650 nm，标准强度0.1 μmol m^-2 s^-1 PAR；调制频率0.6或20 kHz，自动转换。
光化光：
 红色LED，665 nm，最大连续光强600 μmol m^-2 s^-1 PAR
 卤素灯，8V/20W，最大连续光强8500 μmol m^-2 s^-1 PAR
饱和脉冲：卤素灯，8V/20W，最大饱和闪光强度μmol m^-2 s^-1 PAR。
远红光：LED，730 nm，最大强度15 W m-2。
信号检测：PIN-光电二极管，带短波截止滤光片（λ>710 nm）；选择性锁相放大器（专利设计）。
数据存储：128 MB
测量参数：Fo, Fm, Fm’, F, Fo’, Fv/Fm(max. Yield), ΔF/Fm’(Yield), qP, qN, NPQ, ETR, PAR和叶温等。

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